The present invention relates to method, polynucleotides needed for being used to steadily integrate one enter a plant cell, and using including substations material includes primary vector one target sequence of identification for encoding the nucleotide sequences of TAL protein designs It include it is not (i) to be operably linked to an auxiliary agent (" without auxiliary agent flag sequence component ") in secondary vector comprising convert plant in the case where a sequence marker gene corresponding to target sequence (ii), polynucleotides needed for one Identify transgenic plant material, needed for polynucleotides steadily integrated.
