A method for application of an innate immune modulating molecule complex to activate the innate immune system of aquatic animals includes selecting a recombinant DNA with characteristics configured for activating aquatic animal macrophages, such as by inducing an innate immune response in the animal. The method for application of an innate immune modulating molecule complex also includes selecting an adjuvant with characteristics favorable for activating aquatic animal macrophages and causing the release of cytokines. The stimulant recombinant DNA is combined with the adjuvant to form an innate immune modulating molecule (I2M2 complex). The I2M2 complex induces a synergistic response in macrophages that causes activation and proliferation of macrophages, expulsion of or inhibition of pathogens from the macrophages, and release of cytokines that aid in normal innate and acquired immune responses of fish.
