Some embodiments are directed to monoclonal antibodies (mAbs) that bind to transferrin (TF) and transferrin receptor 1 (TFRC), hybridoma lines that secrete these antibodies, and the use of these antibodies to detect TF and TFRC antigens. Some other embodiments are directed to methods and uses for detecting cancer and iron deficiency anemia, as well as methods and uses for distinguishing between early and late stage prostate cancer.