591037 Disclosed are methods of improving tissue wound healing comprising the administration of choroid plexus (CP) secreted proteins isolated or purified from CP condition media to a non-human patient in need thereof. Further disclosed are cosmetic method for improving the appearance of skin after superficial skin wounding, treating a wound, promoting wound healing, decreasing accumulation of collagen or fibroblasts at a wound site or improving hair growth by the administering CP secreted proteins isolated or purified from CP conditioned media. Further disclosed is the use of CP secreted proteins isolated or purified from CP conditioned media in the manufacture of a medicament for improving tissue wound healing, treating a wound, promoting wound healing or decreasing accumulation of collagen or fibroblast at a wound site. Further disclosed is the use of CP condition media or encapsulated CP cells in the manufacture of a medicament for improving tissue wound healing.