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Method for creating perfusable microvessel systems
专利权人:
Thomas Neumann
发明人:
Thomas Neumann
申请号:
US12541707
公开号:
US08846307B2
申请日:
2009.08.14
申请国别(地区):
US
年份:
2014
代理人:
摘要:
Creating a tissue structure in vitro includes juxtaposing mandrels on a culture/perfusion device frame where the mandrels are spaced apart substantially parallel to each other and connecting the mandrels to tubes including an upstream tubes and downstream tubes. The upstream tubes are connected with an upstream manifold and the downstream tubes are connected to a downstream manifold. The frame and the mandrels are sterilized, coated and seeded with cells that multiply and form circular layers around each of the mandrels until the circular layers merge into a tissue structure which is subjected to a growth medium. The mandrels are extracted and the tissue structure is perfused.
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