PROBLEM TO BE SOLVED: To provide a production method of a periodontal ligament cell.SOLUTION: There are provided a differentiation induction method from a mesenchymal stem cell to a ligament cell or a periodontal ligament cell, a production method of a ligament cell or a periodontal ligament cell, and a production method of an implant with a periodontal ligament or a reproduction tooth using the induced periodontal ligament cell, in which, the mesenchymal stem cell and a skin or mucosa-derived stem cell are co-cultured.SELECTED DRAWING: NoneCOPYRIGHT: (C)2020,JPO&INPIT【課題】歯根膜細胞の製造方法の提供。【解決手段】間葉系幹細胞と皮膚又は粘膜由来幹細胞とを共培養することを特徴とする、間葉系幹細胞から靭帯細胞又は歯根膜細胞への分化誘導方法、靭帯細胞又は歯根膜細胞の製造方法、並びに誘導歯根膜細胞を用いた歯根膜付きインプラントおよび再生歯の製造方法。【選択図】なし
