The composition and modification of genomic DNA sequence are provided. These methods generate two phase chromatograms (DSBs) in a predetermined range of genomic DNA sequences, resulting in mutations, insertions and / or displays of DNA sequences in the range of genomic DNA. These components include DNA structures, including nucleoside sequences, which link a cms1 protein in operation with a promoter that can operate in related cells. DNA structures can be used to guide gene gene gene gene gene gene modification. At present,The method of using these DNA structures to change the DNA sequence of genome is explained. In addition, the composition and regulation of gene expression are also provided. These components include DNA structures, including an initiator that can operate in cells that are operationally linked to nucleoside sequences, which encodes a mutated cms1 protein whose ability to produce DSBs has been eliminated, but can optionally be linked to a recording activity domain. These methods can be used to regulate gene expression up and down in the preset genome of Lodge.Se proporcionan composiciones y métodos para modificar secuencias de ADN genómicas. Los métodos producen rupturas bicatenarias (DSBs) en sitios diana predeterminados en una secuencia de ADN genómica, dando como resultado la mutación, inserción y/o deleción de secuencias de ADN en los sitios diana en un genoma. Las composiciones comprenden constructos de ADN que comprenden secuencias de nucleótidos que codifican una proteína de Cms1 operativamente ligada con un promotor que es operable en las células de interés. Los constructos de ADN se pueden usar para dirigir la modificación de ADN genómico en loci genómicos predeterminados. En la presente, se describen métodos para usar estos constructos de ADN para modificar secuencias de ADN genómicas. Adicionalmente, se proporcionan composiciones y métodos para modular la expresión de genes. Las composiciones comprenden constructos de ADN que comprenden u
