Cherng-Kang Perng;Han-Tzo Lin;Shih-Hwa Chiou;Yi-Ping Yang
发明人:
Shih-Hwa Chiou,Cherng-Kang Perng,Han-Tzo Lin,Yi-Ping Yang
申请号：
US13008760
公开号：
US08470597B2
申请日:
2011.01.18
申请国别(地区):
US
年份:
2013
代理人:
摘要：
A composite comprising a stem cell； a biodegradable layer, which can provide an environment for the stem cell to grow and to differentiate, and； a N-isopropylacrylamide (NIPAAm), which can polymerize with the biodegradable layer and possess the temperature-responsive character for easy stripping. The present invention further provides a method for treating a patient with a skin defect, consisting of (a) providing said patient with a composite consisting of a N-isopropylacrylamide (NIPAAm) layer polymerized with a biodegradable layer containing gelatin and a layer of polypropylene (PP) non-woven, wherein a bone marrow derived mononuclear cell with CD45 negative and glycophorin A negative is cultivating on the biodegradable layer； (b) covering said composite on the skin defect of the patient； and (c) treating the composite with water below 25° C. to strip off the layer of polypropylene (PP) non-woven.
