INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY
发明人:
KIM, Du Woon,김두운,KWON, Joseph,권요셉
申请号：
KRKR2017/005390
公开号：
WO2017/204547A1
申请日:
2017.05.24
申请国别(地区):
WO
年份:
2017
代理人:
摘要：
The present invention relates to a method for preparing novel lectin proteins by using a biological method, and has developed a bio-converted novel lectin mixture through a fermentation strain of Bacillus subtilis natto. According to the present invention, the preparation steps and the operation method of a lectin mixture extraction process according to a conventional chemical treatment method have been simplified, and it is confirmed, by using a mass spectrometer, that the fermented products produced according to an optimization method are novel compositions, having different forms, derived from ConA. It is confirmed that the novel compositions have stronger binding force with food poisoning viruses than that of commercial lectin proteins, and thus it is confirmed that the compositions are superior to commercial lectin proteins with respect to virus-neutralizing force. Although it is known that conventional lectin proteins derived from Canavalia gladiata induce hepatotoxicity due to hypersecretion of inflammatory cytokines, the cytotoxicity of a Canavalia gladiata-derived novel composition is respectively reduced by 9% at a concentration of 1 μg/ml and by 8% at a concentration of 10 μg/ml, and thus it is confirmed, at the in vitro level, that hepatotoxicity caused by ConA is alleviated by versatile virus-capturing proteins. Therefore, novelly provided are: versatile virus-capturing proteins, which have strong binding force with food poisoning viruses and reduce hepatocyte toxicity and a preparation method of extraction using fermentation.La présente invention concerne un procédé de préparation de nouvelles protéines de lectine à laide dun procédé biologique, et ayant développé un nouveau mélange de lectine bio-converti par une souche de fermentation de Bacillus subtilis, natto. Selon la présente invention, les étapes de préparation et le procédé de fonctionnement dun procédé dextraction de mélange de lectine selon un procédé de traitement chimique classique ont été
