The present invention relates to a cleaning and sterilization method applied to the objects such as medical equipments, medical instruments for animals, meat processing tools and cooking utensils, which comprises the following steps: a step of alkaline electrolytic water cleaning in which the alkaline electrolytic water is used to perform ultrasonic cleaning or stir cleaning on the aforementioned objects at a first specific time and a step of acidic electrolytic water cleaning in which, following the alkaline electrolytic water cleaning step, the acidic electrolytic water is used to perform stir cleaning or soaking on the aforementioned objects at a second specific time.本發明係一種包括醫療用器具、動物用醫療器具、肉食加工用具及烹調用具之對象物之洗淨殺菌方法,其包括:鹼性電解水洗淨步驟,其利用鹼性電解水於第1特定時間內對上述對象物進行超音波洗淨或攪拌洗淨;及酸性電解水洗淨步驟,其於上述鹼性電解水洗淨步驟後,利用酸性電解水於第2特定時間內對上述對象物進行攪拌洗淨或浸漬。
