The holding device includes a first member, a second member, a third member, a support that supports the first member and supports the second member and the third member relatively movably relative to the first member And an operating section for relatively moving the first member, the second member, and the third member. The first structure can be grasped by the first member and the third member in a state in which the first tissue is disposed between the first member and the second member and between the first member and the third member, With the one member and the third member gripping the first tissue, a second tissue is disposed between the first member and the second member and between the second member and the third member to form the first tissue and the second tissue The first and second tissues can be grasped by the first member and the second member so that the second tissue comes into contact with the first tissue.この保持装置は、第一部材と、第二部材と、第三部材と、第一部材を支持し、第一部材に対して第二部材及び第三部材を相対的に移動可能に支持する支持部と、第一部材と第二部材と第三部材とを相対移動させる操作部と、を備える。第一部材と第二部材との間及び第一部材と第三部材との間に第一組織が配された状態で第一部材と第三部材とによって第一組織を把持可能であり、第一部材と第三部材とが第一組織を把持した状態で、第一部材と第二部材との間及び第二部材と第三部材との間に第二組織を配して第一組織と第二組織とが接するように第一組織および第二組織を第一部材と第二部材とによって把持可能である。