PROBLEM TO BE SOLVED: To provide a novel fish fertilized egg microscopic infusion technique capable of performing efficient and large quantity treatment and being easily handled by any person.SOLUTION: A manufacturing method of fish embryos into which genes are introduced and fish derived therefrom, or the embryos and descendants of the fish comprising the infusion of nucleic acid into the cytoplasm of fertilized fish eggs at the developmental state just after a fertilization and before a germinal disk formation.SELECTED DRAWING: None【課題】効率的かつ大量処理できる、しかも誰でも容易に取り組める新たな魚類受精卵への顕微注入技術を提供すること。【解決手段】受精直後から胚盤形成前の発生段階の魚類受精卵の細胞質中に、核酸を注入することを含む、遺伝子導入された魚類胚又は当該胚由来の魚、或いは当該胚又は当該魚の子孫の製造方法。【選択図】なし