PROBLEM TO BE SOLVED: To provide a new serum-free culture medium for culturing mononuclear cells, and a cultured product.SOLUTION: There are provided a culture method for culturing a mononuclear cell by using serum-free culture medium for culturing mononuclear cells which contains a stem cell factor, a γ-secretase inhibitor, a vascular endothelial cell growth factor, an FMS-like tyrosine kinase 3 ligand and thrombopoietin, and an ischemic disease therapeutic agent and/or an anagenetic therapeutic agent which have a mononuclear cell cultured product in serum-free culture medium as an active ingredient. Further, the ischemic disease therapeutic agent and/or the anagenetic therapeutic agent are a therapeutic agent and an anagenetic therapeutic agent of a disease selected from intractable ulcer, ischemic heart disease, lower-limb ischemic arterial sclerosis, Burger's disease and systemic ischemic disease.SELECTED DRAWING: None【課題】新たな単核球培養用無血清培地及び培養物の提供。【解決手段】幹細胞因子、γ－セクレターゼ阻害剤、血管内皮細胞増殖因子、ＦＭＳ様チロシンキナーゼ３リガンド及びトロンボポエチンを含有する単核球培養用無血清培地を用いて単核球を培養する培養方法、及び無血清培地中の単核球培養物を有効成分とする虚血性疾患治療剤及び／又は組織再生療法剤。また虚血性疾患治療剤及び／又は組織再生療法剤が、難治性潰瘍、虚血性心疾患、下肢虚血性動脈硬化症、バージャー病及び全身の虚血性疾患から選ばれる疾患の治療剤、組織再生療法剤である請求項３記載の虚血性疾患治療剤及び／又は組織再生療法剤。【選択図】なし
