DONG-KI CHOI,SEUNG-MIN SHIN,SUNG-HOON KIM,YONG-SUNG KIM
申请号：
BR112017001306
公开号：
BR112017001306A2
申请日:
2015.07.22
申请国别(地区):
BR
年份:
2018
代理人:
摘要：
method for inhibiting intracellular activated ras using intact immunoglobulin-like antibody capable of penetrating the cytosol and using it, refers to a method for inhibiting intracellular ras using an intact immunoglobulin-like antibody having the ability to penetrate the cytosol, the a heavy chain variable region (vh) that induces an intact immunoglobulin-like antibody to actively penetrate the cytosol of living cells through endocytosis and endosomal escape and bind to the activated ras in the cytosol and an antibody comprising the same, to a a method of inhibiting the growth of cancer or tumor cells and a method of treating cancer or tumor, using the antibody, to a method for screening a variable region of heavy chain that specifically binds ras in the cytosol, to a biologically active molecule fused with the antibody and selected from the group consisting of peptides, proteins, molecule drugs small cell, nanoparticles and liposomes, to a cancer prevention, treatment or diagnosis composition, comprising antibody or active biological molecule fused with the antibody and selected from the group consisting of peptides, proteins, small molecule drugs, nanoparticles and liposomes and the a polynucleotide that encodes the variable region of the light chain and the antibody.método para inibição de ras ativada intracelular utilizando anticorpo tipo imunoglobulina intacta com capacidade de penetração no citossol e utilização do mesmo, refere-se a um método para inibição de ras ativada intracelular utilizando um anticorpo tipo imunoglobulina intacta possuindo a capacidade de penetrar no citossol, a uma região variável de cadeia pesada (vh) que induz um anticorpo intacto do tipo de imunoglobulina para penetrar ativamente o citossol de células vivas através de endocitose e escape endossomal e se ligar a ras ativada no citossol e a um anticorpo compreendendo o mesmo, a um método de inibição do crescimento de células cancerígenas ou tumorais e um método de tratamento de cânce
