The present invention relates to the crystallization of the I types of l-Alanine-(14- Dong Ling grass A prime) ester trifluoroacetate and preparation methods. The preparation method includes crystallizing any crystal form or unformed l-Alanine-(14- Dong Ling grass A prime) ester trifluoroacetate solid in single organic solvent or their mixed organic solvents to obtain the crystallization of l-Alanine-(14- Dong Ling grass A prime) ester trifluoroacetate I types. The crystallization of (14- Dong Ling grass A prime) the ester trifluoroacetate I types of l-Alanine-obtained by the present invention has good stability of crystal form and chemical stability, and recrystallisation solvent low toxicity and low residue used, can preferably use what clinical treatment.本發明涉及L-丙氨酸-(14-冬淩草甲素)酯三氟乙酸鹽的I型結晶及製備方法。所述製備方法包括將任意晶型或無定型的L-丙氨酸-(14-冬淩草甲素)酯三氟乙酸鹽固體在單一的有機溶劑或者他們的混合有機溶劑中結晶得到L-丙氨酸-(14-冬淩草甲素)酯三氟乙酸鹽I型結晶。本發明所得到L-丙氨酸-(14-冬淩草甲素)酯三氟乙酸鹽I型結晶具備良好的晶型穩定性和化學穩定性，並且所用結晶溶劑低毒低殘留，可更好地用於臨床治療。
