A device for separation and collection of tissue from an effluent stream generated in a medical procedure comprises a housing 600 having an upper wall 601a, a lower wall 602a, a circumferential wall 612 extending between the upper wall and lower wall, an inlet aperture 606a for receiving the effluent stream and an outlet aperture 607a for discharge of filtered fluid. The housing encloses a filter member 603a positioned between said upper wall and said lower wall and comprising at least one filter receptacle 615 the upper wall comprising a viewing window 604 for viewing tissue collected in the filter member. The inlet aperture is located beneath the filter member, and the device comprises a fluid pathway 628 for delivering effluent stream received through the inlet aperture into a said filter receptacle from above the filter member. A method of filtering an effluent stream using the device and a kit comprising the device and an illumination device are also included.