SAMUILENKO, Anatoly Yakovlevich,САМУЙЛЕНКО, Анатолий Яковлевич,EREMETS, Vladimir Ivanovich,ЕРЕМЕЦ, Владимир Иванович,ZENOV, Nikolai Ivanovich,ЗЕНОВ, Николай Иванович,LITENKOVA, Irina Yuryevna,ЛИТЕНКОВ
申请号：
RURU2014/000151
公开号：
WO2014/137249A2
申请日:
2014.03.06
申请国别(地区):
WO
年份:
2014
代理人:
摘要：
The invention relates to the field of veterinary virology and biotechnology and can be used for preparing a vaccine against foot-and-mouth disease. In order to increase the purification level of a virus-containing suspension from inactive impurities and to increase the immunogenicity of the desired product in the method for producing the vaccine against foot-and-mouth disease comprising cultivating a viral antigen in a suspension of BHK-21 cell culture at a temperature of 36-37°C, purifying the virus-containing suspension from inactive impurities, inactivating the antigen to the foot-and-mouth virus obtained by concentration, and combining the antigen concentrate with an adjuvant, according to the invention, the purification of the virus-containing suspension from inactive impurities is performed by the addition of polyguanidine derivatives in a final concentration of 0.005-0.01% or of a mixture of chloroform and polyguanidine derivatives in a weight ratios of (40-160):1, respectively, in a final concentration of 0.4-0.8, wherein 1-12-di(guanidine)hexane dihydrochloride, or bis(3-guanidino-propyl)piperazine dihydrochloride, or 3,6-dioxaoctane-1,8-diguanidine dihydrochloride, or 4,9-dioxadodecane-1,2-dibiguanide dihydrochloride is used as the polyguanidine derivatives.Linvention concerne le domaine de la virologie vétérinaire et peut être utilisée dans la fabrication dun vaccin contre la fièvre aphteuse. Pour améliorer le degré de purification de la suspension virale des impuretés de ballast et augmenter limmunogénicité du produit cible, le procédé de fabrication dun vaccin contre la fièvre aphteuse comprend la culture de lantigène viral dans une culture de suspension des cellules VNK-21 à une température de 36-37ºC, la purification de la suspension virale des impuretés de ballast, linactivation de lantigène obtenu par la concentration de lantigène du virus de fièvre aphteuse et la combinaison du concentré de lantigène à un additif, selon linvention, la purification
