The present invention is provided to use RNA to interfere to adjust the compound of expression of target gene, composition and method. RNAi structures of the invention and molecule can be used for being adjusted gene expression with high-caliber RNAi activity and the effect of missing the target reduced or being made silenced gene expression. Advantageous structure includes siRNA targeting any gene, containing one or more 2 -deoxynucleotides in seed zone. The rnai molecule can be used for preventing or treating in the method for disease.
