KIM, HARK KYUN,김학균,PARK, JUN WON,박준원,JANG, SEOK HOON,장석훈
申请号：
KR1020130045644
公开号：
KR1014566270000B1
申请日:
2013.04.24
申请国别(地区):
KR
年份:
2014
代理人:
摘要：
The present invention relates to an animal model of spontaneous gastric cancer and gastric cancer cell lines derived therefrom for the evaluation of the efficacy and toxicity of an immunotherapeutic agent and a gastric cancer therapeutic agent, or for the detection of a metastasis inhibiting gene and the development of a metastasis inhibitor. Specifically, according to the present invention, the animal model of gastric cancer and the gastric cancer cell lines derived therefrom are similar to human gastric cancer in histopathological characteristics, and it has been verified that the sizes of cancer cells of the gastric cancer cell lines were reduced by a 4-1BB inhibitor having anticancer activity. Accordingly, it has been verified that the gastric cancer cell lines can be practically used to evaluate the efficacy and toxicity of an immunotherapeutic agent and a gastric cancer therapeutic agent. In addition, one of the gastric cancer cell lines in the present invention metastasizes quickly so a metastasis-related gene can be detected by making a comparison with other gastric cancer cell lines with respect to gene expression. Furthermore, the metastasis-related gene can be practically used to develop a metastasis inhibitor and evaluate the efficacy of the metastasis inhibitor. Therefore, according to the present invention, it has been verified that the animal model of gastric cancer and the gastric cancer cell lines instead of an immunosuppressive mouse can be practically used to evaluate the efficacy of a gastric cancer inhibitor and a gastric cancer metastasis inhibitor and to evaluate the efficacy of an immunotherapeutic agent.본 발명은 면역 치료 및 위암 치료제에 대한 효능 및 독성 평가 또는 전이 억제 유전자의 발굴 및 억제제의 개발을 위한, 자연발생 위암 동물모델 및 이로부터 수립된 위암 세포주에 관한 것이다. 구체적으로, 본 발명의 위암 동물모델 및 이로부터 수립된 위암 세포주는 조직병리학적 특징이 인체 위암과 유사하고, 항암활성이 있는 4-1BB 억제제에 의해 암세포의 크기가 줄어드는 것을 확인함으로써, 면역 치료 및 위암 치료제에 대한 효능 및 독성 평가에 유용하게 사용될 수 있음을 확인하였다. 또한, 본 발명의 위암 세포주 중 하나는 전이가 빠르기 때문에 다른 세포주들과 유전자 발현을 비교하여, 전이에 관련하는 유전
