A mobile isolation unit which forms a chemical and biological barrier that is has a substantially rectangular-parallelepiped shaped enclosure and a frame system configured to support the enclosure in the suspended position so as to create and internal and external environment. The enclosure may also be equipped with at least one opening for access into and out of the enclosure, a zipper for opening and closing the access port, at least one biomedical port, and a plurality of support points configured to support the enclosed in the suspended position when the frame is in an open position so that a patient can be placed in and out of the enclosure. A method for isolating and treating a patient using the mobile isolation unit is also provided.