A surgical access assembly (100) for positioning within an opening in tissue including an outer frame (110) positionable outside a patient and defining an opening (117) therein dimensioned to receive a surgical instrument therethrough. The outer frame includes a first portion (112), a second portion (130) and a locking portion (114). An inner member (150) is positionable within a patient and a flexible member (170) extends between the inner member and outer frame and is spreadable by actuation of the outer frame.