A peptide-based marker vaccine against Porcine Reproductive and Respiratory Syndrome (PRRS) and a set of immunodiagnostic tests for the prevention, monitoring and control of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) are disclosed. Vaccine formulations according to various embodiments of the invention contain a mixture of peptides derived from PRRSV GP2, GP3, GP4, or GP5 proteins； each peptide individually contains a B cell PRRSV neutralizing/receptor binding epitope which is individually linked to an artificial T helper epitope for enhancement of the respective peptide’s immunogenicity； and which can be supplemented with a mixture of peptides representing the T helper epitopes derived from the PRRSV GP4, GP5, M and Nucleocapsid proteins to provide cell mediated immunity. Such viral peptide compositions are prepared in an acceptable delivery system as vaccine formulations and can provide cross protection of PRRSV antibody free pigs from infection upon PRRSV challenge.本發明揭示一種抵抗豬繁殖及呼吸症候群(PRRS)的胜肽標記疫苗以及一種用於預防、監測與控制豬繁殖及呼吸症候群病毒(PRRSV)的免疫檢測套組。本發明之疫苗組成包含衍自於PRRSV GP2、GP3、GP4或GP5蛋白的胜肽混合物，每個胜肽含有B細胞PRRSV中和/受體結合抗原決定位，其獨立地與人工T輔助細胞抗原決定位結合以促進各胜肽的免疫原性，且其可與含有衍自於PRRSV GP4、GP5、M與核殼蛋白之T輔助細胞抗原決定位的胜肽合併，以提供細胞性免疫。此病毒胜肽組成物於可接受之傳遞系統下製備成疫苗組成，且可對不含PRRSV抗體之豬隻在PRRSV攻毒時提供交叉保護。
