CURTIS, IAN S.,GONZALEZ RAMOS, JAVIER,MIRKOV, T. ERIK
申请号：
CA2836911
公开号：
CA2836911A1
申请日:
2012.06.04
申请国别(地区):
CA
年份:
2012
代理人:
摘要：
The present disclosure relates, in some embodiments, to potato transformation compositions, systems, methods, microorganisms, and plants (e.g., one or more potato chipping varieties). In some embodiments, a method of transforming and/or transfecting a plant (e.g., Atlantic potato) may comprise (a) growing an Atlantic potato plant (e.g., from a tuber) for from about 3 weeks to about 4 weeks, (b) removing one or more leaf sections (e.g., each section from about 0.5 cm to about 1 cm in its longest dimension) from the plant., (c) cultivating the one or more sections on a callus induction medium comprising zeatin for about 2 days, and/or (d) contacting the one or more sections with Agrobacterium comprising the exogenous nucleic acids under conditions that permit transfer of the exogenous nucleic acid to the one or more sections to produce at least one transformed and/or transfected plant cell.
