Abbas Mohammad Ghawi,Ahmed Merzouk,Abdualrahman Abdualkader,Mohammad Alaama
申请号：
US13624860
公开号：
US08784896B2
申请日:
2012.09.21
申请国别(地区):
US
年份:
2014
代理人:
摘要：
Methods are provided for isolating and using a whole-saliva leech extract. The methods can include feeding a phagostimulatory agent to a leech； inducing a regurgitation in the leech, the inducing including placing the leech in an environment having a temperature of less than about 0° C.； and, collecting an unrefined, whole saliva in the regurgitation of the cooled leech. The methods can include revitalizing the leech by warming it at a temperature ranging from about 5° C. to about 40° C. Stable, lyophilized, whole-saliva extracts of a leech are also provided, the extract having a stable activity when stored for use at a temperature below about −20° C., the extract maintaining at least 70% of the activity for at least 6 months. The extracts can be used to treat solid tumors, treat liquid tumors, treat diabetes, treat a viral disease, treat a parasitic disease, treat an antibacterial disease, or serve as an anti-oxidant.
