The present invention has its application in the field of devices and methods for the product physical and chemical mixing and refers particularly to a cartridge for sterile mixing of a two-phase compound. The cartridge includes a first tubular body defining a first collection chamber for a solid phase, a second tubular body defining a second collection chamber for a liquid phase, means for transferring the liquid phase from the second to the first chamber, means for mixing the phases. The means for mixing comprise means for agitating acting on the mixture inside the first chamber with the first tubular body in substantially stationary conditions, so as to favor the dispersion of the solid phase inside the liquid phase thus obtaining a compound with uniform physical and mechanical properties.