An extraction method of birds nest extract comprises following steps. First, a solution of pulverized birds nest is provided into a reaction tank for emulsification. The tank is vacuumed and heated to a first temperature for a first time period so as to form an emulsion of bird’s nest. The tank comprises an emulsifier and a blender which is disposed surrounding the emulsifier. The first temperature ranges from 30 to 60 Celsius degree, and the first time period ranges from 30 minutes to 12 hours. Second, after the temperature of the emulsion of bird’s nest in the tank is adjusted to a second temperature, a protein enzyme is added to the tank, and the tank is kept for a second time period under the second temperature whereas the emulsifier and the blender is kept running. The first temperature ranges from 30 to 40 Celsius degree, and the first time period ranges from 1 to 12 hours. Third, the protein enzyme is deactivated. Fourth, the emulsion of bird’s nest is filtered to obtain a filtered solution of bird’s nest. Fifth, the filtered solution is concentrated and then sterilized so as to obtain a solution of bird’s nest extract.本發明提供一種燕窩萃取物的萃取方法,包含有以下步驟:提供燕窩粉碎物水溶液置於反應槽中進行乳化,加熱至第一溫度並將反應槽抽取真空並持續第一時間,以形成燕窩乳化液,其中反應槽具有乳化器及環繞乳化器之攪拌器,第一溫度為攝氏30至60度,且第一時間為30分鐘至12小時;在維持該乳化器及該攪拌器之運轉之情況下,將燕窩乳化液調整至第二溫度後,將蛋白酵素加入反應槽,並維持於第二溫度下持續第二時間,其中第二溫度為攝氏30度至攝氏40度,且第二時間為1至12小時;終止蛋白酵素之反應;過濾燕窩乳化液,取得燕窩濾液;濃縮燕窩濾液,並將濃縮後之燕窩濾液進行滅菌處理,以取得燕窩萃取液。S1、S2、S3、S4、S5、S6‧‧‧步驟
