Michael Ernest Marino,Faisal Ahmed Syud,Jason William Castle,Brian Duh-Lan Lee,Malin Lindborg,Elin Gunneriusson,Christofer Lendel
申请号：
US12002972
公开号：
US08937047B2
申请日:
2007.12.19
申请国别(地区):
US
年份:
2015
代理人:
摘要：
Provided herein are methods of reducing liver uptake in vivo of a polypeptide that specifically binds to a target comprising: (a) providing a polypeptide that specifically binds to a target and (b) substituting at least one basic or at least one neutral amino acid residue of the native polypeptide from step (a) with an acidic amino acid residue to produce a modified polypeptide, wherein the modified polypeptide demonstrates an isoelectric point at least 0.05-0.1 pH points less than the isoelectric point of the native polypeptide. Also provided are polypeptides made using the inventive methods as well as imaging techniques that employ the methods and agents.
