Федеральное государственное бюджетное учреждение "Медико-генетический научный центр" Российской академии медицинских наук (RU)
发明人:
Гольдштейн Дмитрий Вадимович (RU),Бухарова Татьяна Борисовна (RU),Волков Алексей Вадимович (RU),Логовская Лилия Вячеславовна (RU),Вихрова Екатерина Борисовна (RU),Махнач Олег Владимирович (RU),Логунов
申请号：
RU2012144498/15
公开号：
RU2012144498A
申请日:
2012.10.19
申请国别(地区):
RU
年份:
2014
代理人:
摘要：
1. Biotransplantat for treating bone degenerative diseases, characterized in that it comprises a multipotent stromal cells (MSCs) derived from fat tissue from adult donors transfected gene BMP2, characterized immunophenotype CD13 +, CD29 +, CD44 +, CD73 +, CD90 +, CD105 +, CD31 -, CD34-, CD45-, capable of directed differentiation into osteogenic, chondrogenic and adipogenic directions and a biocompatible carrier, which is a highly purified bone matrix with a stored and collagen and a mineral component n irodnoy arhitektonikoy.2. Biotransplantat according to claim 1, characterized in that the biocompatible carrier material is used "Osteomatrix" 3. Biotransplantat according to claim 1, characterized in that multipotent stromal cells obtained from adipose tissue from adult donors, including for autologous use, by lipoaspiratsii.4. A process for preparing biotransplantata for the treatment of degenerative bone diseases according to claim 1, characterized in that lipoaspirate obtained from adult donors, disaggregate by incubation in Versene solution with addition of 0.25% trypsin at 37 ° C for 2 hours, after centrifugation, the pellet diluted growth medium with 10% fetal calf serum and 100 mg / l amikacin, and cells were cultured on petri dishes under standard culture conditions: 37 ° C, 5% CO saturating humidity conditions derived MSCs culture prior to transfection astyat to 80% of the monolayer in complete growth medium, then prepared adenoviral construct carrying BMP-2 cDNA of bone morphogenetic protein, wherein the synthesis of the complete cDNA copy of the gene with BMP-21. Биотрансплантат для лечения дегенеративных заболеваний костной ткани, характеризующийся тем, что содержит мультипотентные стромальные клетки (МСК), полученные из жировой ткани взрослых доноров, трансфицированные геном ВМР-2, характеризующиеся иммунофенотипом CD13+, CD29+, CD44+, CD73+, CD90+, CD105+, CD31-, CD34-, CD45-, способные к направленной дифференцировке в остеогенном, хондрогенном и адипо
