To provide microbial oil containing dihomo-γ-linolenic acid as a constituent fatty acid in which the content ratio by weight of arachidonic acid to dihomo-γ-linolenic acid is less than 1/100.SOLUTION: According to the invention, a microbial oil containing dihomo-γ-linolenic acid is produced by culturing a microorganism of which Δ5 desaturase activity is lowered or deleted in a culture liquid added with at least two Δ5 desaturase inhibitors.SELECTED DRAWING: None【課題】油の構成脂肪酸としてジホモ－γ－リノレン酸を含み、ジホモ－γ－リノレン酸に対するアラキドン酸の含有率が、重量比（アラキドン酸／ジホモ－γ－リノレン酸）で１／１００未満である微生物油の提供。【解決手段】培養液に、少なくとも２種類のΔ５不飽和化酵素阻害剤を添加、前記培養液中で、Δ５不飽和化活性が低下又は欠失した微生物を培養して、ジホモ－γ－リノレン酸含有微生物油を製造する。【選択図】なし
