A method of production of a stable composite of bacterial bionanocellulose with perforated metal or polymeric material, designed for tissues reconstruction
The method of production of a stable composite of bacterial bionanocellulose with perforated metal or polymeric material, designed for reconstructive surgery of soft and hard tissues, that implicates the placement of a sterile, perforated polymeric or metallic material in a cultivation medium of Guconacetobcter xylinus bacterial strain and conduction of a stationary culture, is characterized by the vertical fixation of polymeric or metallic perforated material designated to be overgrown with cellulose membrane during the cultivation in a bioreactor. The materials lower edge is suspended in a culture medium for the first 2-3 days, i.e. until the first layers of cellulose are formed, and in the next days it is successively dropped down into the medium until it is totally covered with cellulose membrane. The overgrown material is further taken off from the bioreactor, excised from the excess of the membrane, purified, pressed up to the proper thickness, packed and sterilized. There is one or more polymeric or metallic perforated materials that may be fixed vertically in the bioreactor at a properly adjusted distance from each other and are overgrown simultaneously by cellulose membrane.
