EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA - EMBRAPA;DE MELLO BRANDAO, HUMBERTO;DE MELLO BRANDÃO, Humberto;EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA - EMBRAPA
发明人:
DE MELLO BRANDAO, HUMBERTO,DE MELLO BRANDÃO, Humberto
申请号：
BRBR2011/000400
公开号：
WO2012/054999A1
申请日:
2011.10.27
申请国别(地区):
BR
年份:
2012
代理人:
摘要：
The present invention relates to a method for producing and using compositions containing propolis nanoparticles, optionally combined with one or more substances of interest, such as active principles, and also, optionally, substances which have a secondary effect, such as adjuvants and synergistic agents. The method for producing said nanoparticles comprises the following steps: i) preparing a fraction A, which consists of a propolis extract dissolved in an organic solvent, one or more stabilizers and/or emulsifiers, optionally, being added to said fraction A, as well as, optionally, one or more substances of interest and/or that have a secondary effect； ii) preparing a fraction B, which is an aqueous phase, composed of: (ii.1) water, or (ii.2) a solution or aqueous dispersion, one or more stabilizers and/or emulsifiers, optionally, being added to said fraction B； iii) adding, drop by drop, fraction A to fraction B or fraction B to fraction A； iv) homogenizing the mixture by means of mechanical stirring, a dispersion of nanoparticles with an average size of 1 to 1000 nm being spontaneously formed； v) optionally including the following additional steps: (v-1) removing the organic solvent, and/or (v-2) drying the nanodispersion. The compositions developed can be used within, for example, the agrochemical, pharmaceutical, cosmetic or chemical industries.La présente invention concerne un procédé d'obtention et d'utilisation de compositions constituées de nanoparticules de propolis, éventuellement associées à une ou plusieurs substances d'intérêt, telles que des principes actifs, ainsi que, éventuellement, des substances à effet secondaire, telles que des synergistes et des adjuvants. Le procédé d'obtention des nanoparticules comprend les étapes suivantes: (i) préparation d'une fraction A, consistant en un extrait de propolis dissous dans un solvant organique, à laquelle un ou plusieurs stabilisants et/ou émulsifiants peuvent être additionnés, une ou plusieurs substance
