Methods and systems of analyzing retinal nerve fiber layer for detecting glaucoma and/or other retinal nerve fiber indicated eye conditions include collecting retinal nerve fiber layer image data, segmenting the retinal nerve fiber layer image data by removing retinal nerve fiber layer image data below a threshold thickness value, determining an area or a volume of the thickness of the retinal nerve fiber layer in the retinal region of interest and determining a severity of the glaucoma and/or at least one other retinal nerve fiber indicated eye condition for the subject.
