The invention provides a method for enhancing lipid production in microalgae comprising culturing the green microalgae in osmotic stress. The invention also provides methods for producing bio-diesel, food and feed.本發明係提供一種加速微細綠藻油脂合成之方法,其包含使微細綠藻受滲透壓逆境。本發明亦提供生產生質柴油、食品及飼料之方法。(無元件符號說明)