The present application provides a linear stapler with an adjusting mechanism, comprising an adjusting shift lever with at least one step surface on each of an upper end and a lower end, an adjusting slider with at least three different step surfaces on an end surface close to the adjusting shift lever, and an adjusting chassis having at least three different step surfaces on an end surface close to the adjusting shift lever； when an adjusting knob is shifted, the adjusting shift lever rotates therewith, so that the adjusting shift lever generates relative displacement in a direction vertical to the step surface with the adjusting slider at one end, and generates relative displacement in a direction vertical to the step surface with the adjusting chassis at the other end, and further drives a nail abutting seat subassembly to generate rotation relative to a nail bin seat, until the thickness of clamped tissue is consistent in the near end and the far end. Compared with adjusting mechanisms in the prior art, the present application has a wider adjusting scope and a more suitable pressing force and is more convenient to operate.
