The method for describing the method for culture autogenous cell and treating mammalian subject.Cell culture is on host material, which has the surface handled with aliphatic ester to have strong hydrophobic surface, and the autologous plasma obtained from the blood sample of patient cultivates cell as growth medium.Host material also serves as the transfer dressing for the cell of culture to be transferred to patient, and is used as wound covering dressing.Invention further describes use the cultural method to cultivate the kit and system of autogenous cell.