PROBLEM TO BE SOLVED: To provide a fluorescence imaging device capable of acquiring a subject distance while suppressing an increase in the number of parts. SOLUTION: This fluorescence imaging apparatus 100 includes visible light illumination 3 and excitation light illumination 4, visible light Vis reflected by a subject P, and near infrared fluorescence IR emitted from a fluorescent agent Pa by excitation light EL. The image pickup section 5 for picking up the image, the image processing section 15 for obtaining the visible light image 17b and the fluorescence image 17a, the visible light illumination 3 and the excitation light illumination 4 are provided separately in the image pickup section 5 and projected onto the subject P. The subject distance WD between the subject P and the imaging unit 5 is acquired based on the projection light source 6 that emits the light SL and the image 18 of the projection light SL that is acquired by the image processing unit 15 and is obtained by the projection light source 6. The subject distance acquisition unit 16 for [Selection diagram] Figure 1【課題】部品点数が増加することを抑制しつつ、被写体距離を取得することが可能な蛍光イメージング装置を提供する。【解決手段】この蛍光イメージング装置100は、可視光照明3と励起光照明4とを含み、被写体Pによって反射した可視光Visと、励起光ELによって蛍光薬剤Paから発せられる近赤外蛍光IRとを撮像する撮像部5と、可視光画像17bおよび蛍光画像17aを取得する画像処理部15と、可視光照明3および励起光照明4とは別に撮像部5に設けられ、被写体Pに対して投影光SLを照射する投影光光源6と、画像処理部15によって取得された、投影光光源6による投影光SLの画像18に基づいて、被写体Pと撮像部5との間の被写体距離WDを取得する被写体距離取得部16と、を備える。【選択図】図1