Provided herein are IL-2 muteins and IL-2 mutein Fc-fusion molecules that preferentially expand and activate T regulatory cells and are amenable to large scale production. Also provided herein are variant human IgG1 Fc molecules lacking or with highly reduced effector function and high stability despite lacking glycosylation at N297. Also, provided herein are linker peptides that are glycosylated when expressed in mammalian cells.本發明提供優先擴展並激活T調節細胞並適用於大規模生產之IL-2突變蛋白及IL-2突變蛋白Fc融合分子。本發明亦提供缺乏或顯著降低效應子功能且儘管缺乏N297處之糖基化但仍具有高穩定性之變體人類IgG1 Fc分子。而且，本發明提供當在哺乳動物細胞中表現時會糖基化之連接體肽。
