The present disclosure relates to a system and a method for automatically measuring and assessing hemodynamics in tissue of an anatomical structure of a subject. In particular the present disclosure relates to continuously measuring and assessing hemodynamics in medical procedures using fluorescence imaging and wherein the administration of the fluorescent agent is controlled and automated. One aspect relates to a method of automatic perfusion assessment of an anatomical structure of a subject, the method comprising administration into a vein of a bolus corresponding to less than 0.01 mg ICG/kg body weight of a first fluorescence imaging agent. Another aspect relates to a system for automatic perfusion assessment of an anatomical structure during a medical procedure of a subject comprising a controllable injection pump for holding at least one first fluorescence imaging agent, the injection pump being configured for injecting a predefined amount of said first fluorescence imaging agent into the blood of the subject, wherein the system is configured for receiving and analysing a time series of fluorescence images of the tissue of said anatomical structure following the injection of the first fluorescence imaging agent, and determining at least one perfusion parameter of said anatomical structure based on said analysis.La présente invention concerne un système et un procédé pour mesurer et évaluer automatiquement l'hémodynamique dans un tissu d'une structure anatomique d'un sujet. En particulier, la présente invention concerne la mesure et l'évaluation en continu de l'hémodynamique dans des interventions médicales à l'aide d'une imagerie par fluorescence et l'administration de l'agent fluorescent étant régulée et automatisée. Un aspect concerne un procédé d'évaluation automatique de la perfusion d'une structure anatomique d'un sujet, le procédé comprenant l'administration, dans une veine, d'un bolus correspondant à moins de 0,01 mg d'ICG/kg du poids corporel d'un prem
