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Strains of agrobacterium modified to increase plant transformation frequency
专利权人:
DOW AGROSCIENCES LLC
发明人:
MERLO DONALD J.,RUSSELL SEAN M.,RETALLACK DIANE,WOOSLEY AARON,MEADE TOM,NARVA KENNETH E.
申请号:
NZ60558411
公开号:
NZ605584A
申请日:
2011.07.29
申请国别(地区):
NZ
年份:
2014
代理人:
摘要:
Discloses a method for transforming a plant, comprising contacting a cell of the plant with an agrobacterium strain having at least one pTi helper plasmid comprising a 14.8 Kpnl fragment of pSB1 that is deficient in RecA function and a pTi plasmid having at least one disarmed T-DNA region, the T-DNA region comprising at least a right T-DNA border and exogenous DNA adjacent to the border, wherein the plasmids have differing origins of replication relative to each other. Also discloses the Agrobacterium and plant.
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