PROBLEM TO BE SOLVED: To provide a method for contributing to the cancer treatment with lighter side effects by an action mechanism different from an anticancer agent having toxicity also to the normal tissue.SOLUTION: An siRNA (siRNA of C2Gnt), which inhibits RNA synthesis of glycosyltrasferase (core2-N-acetylglucosaminyltransferase-I:C2GnT-I) for synthesizing malignancy-related sugar chain core2O-glycan in cancer cells, is used in a cancer treatment agent. siRNA can be produced in cells by introducing vectors of plasmids, etc., into which the DNA sequence of siRNA of C2GnT and the DNA sequence of antisense thereof are introduced, into the cells.COPYRIGHT: (C)2012,JPO&INPIT【課題】正常組織に対しても毒性を有する抗癌剤とは異なる作用機序によって、より副作用の軽い癌治療に資する方法を提供する。【解決手段】癌細胞の悪性度関連糖鎖コア2Ο-グリカンを合成する糖転移酵素core2-N-acetylglucosaminyltransferase-I:C2GnT-I)のRNA合成を阻害するsiRNA(C2GnTのsiRNA)を癌治療剤に用いる。C2GnTのsiRNAのDNA配列、およびそのantisenseのDNA配列が導入されたプラスミド等のベクターを細胞に導入して、細胞内でsiRNAを産生することができる。【選択図】図1
