POSTECH ACADEMY-INDUSTRY FOUNDATION ;THE REGENTS OF THE UNIVERSITY OF MICHIGAN
发明人:
PARK Jae Sung,LABUZ Joseph M,TAKAYAMA Shuichi,SHIN Hyun Woo,HAN Chung Min
申请号：
US201615562549
公开号：
US2018164197(A1)
申请日:
2016.03.03
申请国别(地区):
美国
年份:
2018
代理人:
摘要：
Disclosed is a method of isolating extracellular vesicles using an aqueous two-phase system (ATPS), including (a) preparing an ATPS by mixing a first material and a second material, which are immiscible with each other, with a body fluid or an aqueous solution containing extracellular vesicles and (b) isolating extracellular vesicles concentrated in the second material of the ATPS. This method can exhibit very high isolation efficiency, a simple isolation manner, and a very short isolation time. The isolation of extracellular vesicles using the ATPS requires no ultracentrifuge and achieves almost 100% isolation efficiency within a short time of about 10˜20 min, and thus the method of the invention is practical, is economical due to low costs thereof, can increase the purity of extracellular vesicles contaminated with protein, enables the diagnosis of disease using the isolated extracellular vesicles, and can be applied to various fields using extracellular vesicles.
