PROBLEM TO BE SOLVED: To provide antigen-binding molecules exhibiting enhanced uptake into cells of an antigen, antigen-binding molecules that accelerate the reduction of antigen concentration in plasma, antigen-binding molecules capable of binding to an antigen a plurality of times, antigen-binding molecules having improved pharmacokinetics, pharmaceutical compositions comprising the antigen-binding molecules and production methods thereof.SOLUTION: Disclosed herein is a method for producing an antigen-binding molecule, which comprises the steps of: (a) providing a sequence of an antigen binding molecule comprising an antigen-binding domain and an FcRn-binding domain； (b) identifying in the sequence, a sequence that can be a candidate for a motif of sugar chain receptor-binding domain； (c) designing a motif of sugar chain receptor-binding domain whose sequence is different from the sequence identified in the step (b) by at least one amino acid； (d) preparing a gene encoding the polypeptide of the antigen binding molecule comprising the motif of the sugar chain receptor-binding domain designed in the step (c)； and (e) recovering the antigen binding molecule from the culture fluid of a host cell transformed with the gene obtained in the step (d).SELECTED DRAWING: None【課題】抗原の細胞内への取込が促進された抗原結合分子、血漿中の抗原濃度の減少を促進させる抗原結合分子、複数回抗原に結合できる抗原結合分子、薬物動態が改善された抗原結合分子、当該抗原結合分子を含む医薬組成物、及びそれらの製造方法の提供。【解決手段】以下の工程を含む抗原結合分子の製造方法；(a)抗原結合ドメイン、FcRn結合ドメインを含む抗原結合分子の配列を提供する工程、(b)当該配列中における糖鎖受容体結合ドメインのモチーフの候補となる配列を同定する工程、(c)(b)で同定された配列と少なくとも１つのアミノ酸が異なる配列を含む糖鎖受容体結合ドメインのモチーフを設計する工程、(d)(c)で設計された糖鎖受容体結合ドメインのモチーフを含む抗原結合分子のポリペプチドをコードする遺伝子を作製する工程、(e)(d)で得られた遺伝子によって形質転換された宿主細胞の培養液から抗原結合分子を回収する工程。【選択図】なし
