Thomas J. Kelleher,Jan-Ji Lai,Joseph P. DeCourcey,Paul D. Lynch,Maurizio Zenoni,Auro R. Tagliani
申请号：
US14475319
公开号：
US20140371135A1
申请日:
2014.09.02
申请国别(地区):
US
年份:
2014
代理人:
摘要：
The invention discloses highly purified daptomycin and to pharmaceutical compositions comprising this compound. The invention discloses a method of purifying daptomycin comprising the sequential steps of anion exchange chromatography, hydrophobic interaction chromatography and anion exchange chromatography. The invention also discloses a method of purifying daptomycin by modified buffer enhanced anion exchange chromatography. The invention also discloses an improved method for producing daptomycin by fermentation of Streptomyces roseosporus. The invention also discloses high pressure liquid chromatography methods for analysis of daptomycin purity. The invention also discloses lipopeptide micelles and methods of making the micelles. The invention also discloses methods of using lipopeptide micelles for purifying lipopeptide antibiotics, such as daptomycin. The invention also discloses using lipopeptide micelles therapeutically.
