Disclosed is an amphichrome cultivation device, comprising a cultivation slot and a planting plate, wherein the planting plate and the cultivation slot are used separately, or the planting plate covers the cultivation slot and is adapted, together with the cultivation slot, to form a cavity for containing a nutrient solution or for accommodating a nutrient aerosol, so as to provide a dark growth environment for roots and make insufficient light available for algae in the nutrient solution or the nutrient aerosol, so that the reproduction and growth of same are impossible. At least one of an inner side wall of the cultivation slot, an inner bottom surface of the cultivation slot and a lower surface of the planting plate is completely covered by at least one light blocking layer, and a preparation method for the light blocking layer comprises coating, injection molding, laminating, bonding and spraying. The cultivation device of the present invention can minimize the light intensity under the planting plate, pr
