A percutaneous spine puncture guiding system includes a puncture guiding mechanism which guides a puncture needle to perform a percutaneous puncture, an X-ray image capture device for capturing two X-ray images of the side and the back of a patient, and a puncture orientation calculation sub-system which obtains, based on a path in the X-ary images as desired by a medical staff member, puncture orientation data suitable for adjustment of the puncture guiding mechanism so as to guide a guiding ball of the puncturing needle. The percutaneous spine puncture guiding system can guide the puncture needle stably and precisely with no need to generate much more than two X-ray images used to continuously check puncture sites of the puncture needle, thereby minimizing the number of X-ray image-capturing during a surgical operation. Therefore, such advanced percutaneous spine puncture guiding system can also be used to embody an inventive puncture orientation configuring method.一種脊椎經皮穿刺導引系統與穿刺方位規劃方法,該系統包含一導引一穿刺針經皮穿刺的穿刺導引機構、一用以擷取患者之背部與側部的兩X光影像的影像擷取裝置,及一穿刺方位計算子系統。該穿刺路徑計算子系統能依據一醫護人員於該等X光影像中標示的路徑,規劃出便於該醫護人員調整該穿刺導引機構導引該穿刺針之一導引球的穿刺方位資料。該系統除了能穩定且準確地導引該穿刺針外,還因無需多次拍攝X光影像以不斷確認穿刺狀態,故能減少手術過程中的X光影像拍攝次數,是一種創新的脊椎經皮穿刺導引系統並使用了嶄新的穿刺方位規劃方法。4‧‧‧角度計42‧‧‧角度感測模組43‧‧‧無線通訊模組5‧‧‧影像擷取裝置6‧‧‧穿刺方位計算子系統61‧‧‧顯示單元62‧‧‧路徑規劃單元63‧‧‧空間關係分析單元64‧‧‧穿刺方位計算單元