A method for Diffuse Optical Tomography (DOT) reconstruction by overlapping/compose of sub-images, the method include steps: (a) build at least one mode, in the mode there’re several sub-regions in a frame (b) find a diffusion matrix for each sub-region (c) using a forward model to find weighting matrix for each sub-region, and using TJSVD to solve the inverse weighting matrix (d) multiply each diffusion matrix by each inverse weighting matrix and get the absorption coefficients of matrix for each sub-regions and (e) compose the absorption coefficients of matrixes and add up the non-overlapped sub-images and averaging the overlapped sub-images.一種擴散光學斷層掃瞄之重疊/組合影像重建方法,包含下述步驟:(a)建立至少一在預定範圍選擇多個待處理的子區域的處理模式;(b)對各子區域取得一部分光線強度矩陣;(c)對各子區域以前算模型取得一部分權重矩陣,並利用奇異分解法換算一部分權重逆矩陣;(d)將各子區域之部分光線強度矩陣與部分權重逆矩陣相乘得到各子區域之部分吸收係數矩陣;及(e)將各子區域之部分吸收係數矩陣依位置重疊/組合。201~212...步驟