The invention provides a newflammulina veluptipesspecies whose fruit body can be induced at room temperature and the culturing method thereof. This species can be cultured and grown at room temperature, so it is unnecessary to reduce temperature in order to induce the growth of its fruit body. After erasing the hyphae film on the surface of the medium in a culture vessel and adding an appropriate amount of sterile water, the fruit body can be induced at room temperature.本發明提供一種可在常溫下出菇之新穎金針菇(Flammulina velutipes)菌種,及其培養方法。該金針菇菌種可於常溫下培養長成菌絲後,不用降低溫度刺激出菇,直接在室溫下刮除培養瓶上方之菌絲膜,添加適量的無菌水後,即可在室溫下出菇。