PURPOSE: A nanocomposite for reconstruction or regeneration of chondrocytes and a method for differentiating stem cells into chondrocytes are provided to effectively deliver hSOX9 gene and siRNA to mesenchymal stem cells. CONSTITUTION: A nanocomposite for reconstruction or regeneration of chondrocytes is prepared by binding hSOX9 gene or nucleotide siRNA containing a base sequence of sequence numbers 1-6 on nanoparticles of biodegradable polymers. The biodegradable polymers are a poly(lactic-co-glycolic acid). The weight ratio of the hSOX9 gene and biodegradable polymers is 1:40-100. A method for differentiating mesenchymal stem cells into chondrocytes comprises a step of culturing the mesenchymal stem cells under the presence of the nanocomosite. A pharmaceutical composition for reconstruction or regeneration of chondrocytes contains the nanocomposite-introduced mesenchymal stem cells and pharmaceutically acceptable carrier.본 발명은 본 발명은 생분해성 고분자의 나노입자 상에, hSOX9 유전자 및 특정 siRNA를 결합시켜 얻어진 연골조직 재건 또는 재생을 위한 나노 복합체 및 이를 이용한 중간엽 줄기 세포의 연골세포로의 분화방법을 제공한다. 또한, 본 발명은 상기 나노 복합체가 도입된 중간엽 줄기 세포를 포함하는 연골조직 재건 또는 재생을 위한 약학 조성물, 즉 세포 치료제를 제공한다.
