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Method for cultivation of genetically-modified plant
专利权人:
Saori Kasahara
发明人:
Saori Kasahara,Masafumi Wasai,Koichi Sugita,Teruhisa Shimada
申请号:
US13147436
公开号:
US09353379B2
申请日:
2009.08.26
申请国别(地区):
US
年份:
2016
代理人:
摘要:
The present invention provides a method for cultivation of a genetically-modified plant that can highly produce a desired protein. More specifically, the present invention provides a method for cultivation of a genetically-modified plant comprising: cultivating the genetically-modified plant in a medium,wherein the genetically-modified plant is transformed by introducing an expression vector comprising a promoter regulating expression of RNA and a seed storage protein isolated from a plant that highly expresses RNA and the seed storage protein under a high nitrogen condition and a polynucleotide encoding an objective protein, andwherein the medium is adjusted so that nitrate nitrogen is 70 mg/L to 750 mg/L and/or ammonium nitrogen is 70 mg/L to 750 mg/L for a definite period in a period from 30 days before an expected flowering date to a date on or before flowering of the genetically-modified plant.
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