The improvement of physiological activity inducing substance intracellular (Bio-active Induce matter:BAIM) is cultivated and extracted to a kind of rhizopus (Rhizopus SPP),To put Oscillation culture Hou on the 5th in 30 DEG C of insulating boxs of what in the culture medium of rhizopus (Rhizopus SPP) culture what Improvement type liquid (Sabourand Dextrose Agar),0.2ml bacterium solution is taken to be applied in an Improvement type solid (Sabouraud Dextrose Agar) culture medium,It stands in 30 DEG C of insulating boxs of what and cultivates 5,White colony is scraped before not occurring black colonies,And put in what Sterile phosphate buffer solution (Phosphate Buffer Solution PBS) 10ml,Suspension Hou carry out rhizopus extraction intracellular again. It is that 10 minutes (4 DEG C) Hous are centrifuged with head mold bacterium suspension 10000xg revolution to take its bacterial sediment object again that it, which is extracted, Sterile phosphate buffer solution 10ml is added again at suspension, it is centrifuged again, continuously repeat Hou three times, again by complete flushing bacterial sediment object with weight 1:9 (bacterial sediment object: PBS buffer solution) ratio again at suspension, it is carried out continuously the processing routine for freezing (- 80 DEG C) and defrosting (37 DEG C of water-baths) three times, smashing device again with ultrasonic cell, to carry out broken wall Hou (primary at interval of 5 minutes broken walls under water-bath, it is carried out continuously 5 times). Again by the suspension bacteria liquid of broken wall completion again with the centrifuge separation of 10000xg revolution 10 minutes (4 DEG C or less) Hou, take its supernatant with 0.45 μm of membrane filtration, its most Hou filtrate contains head mold thallus physiological activity inducing substance (BAIM) intracellular, dry up to this case product (physiological activity secretion inducing substance) through the spraying universe. It, which can induce, pro
